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Image Search Results
Journal: Cell reports
Article Title: Candidate stem cell isolation and transplantation in Hexacorallia.
doi: 10.1016/j.celrep.2024.114944
Figure Lengend Snippet: Figure 2. Recipient rescue and transplanted cell longevity (A and B) For survival assays, non-transplanted animals were treated with a lethal dose of MITC (75 mM) and 3 days later injected with donor cells. Their viability was scored weekly. (A) Survival curve of one representative experiment of 11 transplanted and 10 non-transplanted animals. (B) Four rescue experiments were repeated to yield 34 animals in the transplanted group and 32 in the non-transplanted group. The fraction of surviving animals 4–8 weeks after transplantation was calculated (*p = 0.0103, Fisher’s exact test). (C and D) For longevity, WT recipient animals were treated with a sublethal dose of MITC and then injected with TBP::mCherry donor cells. T 6–8 weeks after the transplantation, the recipient animals were dissociated, and their mCherry+ cells were collected by FACS. A total of 40,000 of these were then transplanted again into sublethally MITC-treated recipient animals. Twelve transplanted animals and 15 non-transplanted animals (see Figure S3A) were incubated and scored weekly for mCherry signal for 6 weeks (C), a representative experiment. (C) A representation of the percentage of animals expressing the mCherry signal over time after the longevity transplantation experiment (n = 6 in each group). (D) Confocal microscope images illustrating the mCherry signal in a transplanted animal, 31 days after serial transplantation (D, top) and a non-transplanted animal under the same conditions (D, bottom; scale bar, 100 mm).
Article Snippet: The transplantation system is composed of a
Techniques: Injection, Transplantation Assay, Incubation, Expressing, Microscopy